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KMID : 0352419940130040443
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Keimyung Medical Journal
1994 Volume.13 No. 4 p.443 ~ p.446
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Detection of Mycobacterium Tuberculosis DNA in a Case of Lupus Vulgaris Using a Nested-Primer Gene Amplification Assay
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신문석
권호준/김병천/이규석/송준영/전효진/김재룡
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Abstract
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nstration of M. tuberculosis directly or in culture in some of these eruptions can be difficult. It is essential to demonstrate the presence of M. tuberculosis in a clinical sample for a definitive diagnosis of tuberculosis.
With the discovery of the polymerase chain reaction (PCR), the DNA diagnostic approach for infection has developed in a short period. With the dual purpose of definitive and rapid diagnosis of tuberculosis, this study examined the usefulness of a
nested
PCR for detection of M. tuberculosis. Compared with a single-step nested PCR, the two-step PCR was able to enhance sensitivity approximately 1, 000-fold on the basis of bacterial counts and DNA quantity
We concluded that two-step nested PCR appeared to be the most useful PCR protocol for early and sensitive diagnostic method detecting M. tuberculosis in acid-fast stain negative specimens.
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KEYWORD
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